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    Home > Biochemistry News > Biotechnology News > Deletion of a single allele of the gene encoding Zbtb38 causes early embryonic death

    Deletion of a single allele of the gene encoding Zbtb38 causes early embryonic death

    • Last Update: 2022-05-19
    • Source: Internet
    • Author: User
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    Figure: Heterozygous deletion of Zbtb38 (Zbtb38+/-) reduces epithelial cell proliferation shortly after implantation and increases apoptosis (E4.
    5~), resulting in early embryonic death

    .
    The expression of Nanog and Sox2 was decreased in Zbtb38+/- embryos

    .
    ICM: intracellular clumps

    .


    DNA methylation is an important epigenetic modification that is critical for mammalian development
    .
    For example, DNA methylation is critical for endless biological processes such as gene regulation and cell fate determination

    .
    In mammals, DNA methyltransferases are key to the reconstruction of the overall DNA methylation pattern in blastocysts during implantation

    .
    This is critical for passing epigenetic information to the next generation

    .
    On the other hand, the role of methyl-CpG-binding proteins (MBPs) in binding methylated CpGs as part of the DNA methylation process remains unclear

    .
    However, a previous study by researchers at the Nara Institute of Science and Technology (NAIST) in Japan clarified this; Zbtb38, also known as CIBZ, a zinc-finger MBP, inhibits the expression of mouse embryonic stem (ES) cells.
    Growth is critical

    .
    They further demonstrated that Zbtb38 promotes the expression of Nanog, which is the basis for embryonic stem cell growth

    .
    However, what Zbtb38 does in real life remains a mystery

    .

    To further unravel the mystery, the same team of scientists at NAIST, led by Eishou Matsuda, used the Crep-LOXP technology to create conditional Zbtb38 knockout mice
    .
    Their pioneering study showed that deletion of a single Zbtb38 allele in the germline results in decreased ectodermal cell growth and increased apoptosis shortly after implantation, resulting in early embryonic death

    .
    When Zbtb38 is lost in heterozygous embryos, Nanog, Sox2, and genes that control ectoderm growth and differentiation become dysfunctional

    .

    "Our results show that germline deletion of Zbtb38 monoallelic reduces ectodermal cell proliferation shortly after embryo implantation and increases apoptosis, leading to early embryonic death
    .
    Loss of heterozygous Zbtb38 reduces Nanog, Sox2 and expression of genes involved in ectodermal proliferation, differentiation and cell viability

    .
    This finding suggests that methyl-cpg binding proteins play a role in controlling embryonic phenotype," explains Matsuda

    .

    "For the first time, we have demonstrated a link to embryonic function in a protein that has long been thought to bind methyl-cpg," said co-author Yasumasa Ishida
    .
    "This provides a huge opportunity to further study how Zbtb38 works during embryonic development

    .
    More research needs to be done to elucidate the specific molecular mechanisms.

    Zbtb38 is present in all tissues, and it is associated with height, cancer, neurodegenerative diseases and rheumatoid arthritis, etc.

    Therefore, the creation and analysis of tissue-specific cret-mediated knockout mice will help us understand the physiological function of Zbtb38 and Zbtb38-related diseases," concluded Matsuda
    .

    The findings of this work will be of interest to developmental biologists because it highlights the epigenetic significance of DNA methylation in early pregnancy
    .

    article title

    Heterozygous loss of Zbtb38 leads to early embryonic lethality via the suppression of Nanog and Sox2 expression

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