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    Home > Biochemistry News > Biotechnology News > Demystifying the taste molecular basis of Pieris rapae perceiving myrosin in cabbage

    Demystifying the taste molecular basis of Pieris rapae perceiving myrosin in cabbage

    • Last Update: 2021-07-30
    • Source: Internet
    • Author: User
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    The large number and complex structure of secondary substances in plants constitute the unique taste of each plant
    .


    Most of these secondary substances have a defensive effect on herbivorous insects and hinder their feeding


    Pieris rapae ( Pieris rapae ) is a worldwide important agricultural pest.
    It is addicted to cruciferous vegetables such as cabbage, broccoli, rape, cabbage, and radish, which are eaten by the common people
    .


    The main reason is that the cabbage butterfly is addicted to glucosinolates, a type of secondary substance unique to these plants


    On July 15, 2021, Wang Chenzhu’s team from the Institute of Zoology, Chinese Academy of Sciences published a research paper titled "Identification of a gustatory receptor tuned to sinigrin in the cabbage butterfly Pieris rapae " in PLoS Genetics , revealing for the first time that Pieris rapae feels a kind of The important glucosinolate-the taste receptor of sinigrin
    .

    The team used techniques and methods such as behavior, electrophysiology, cell biology, molecular biology, and omics to study the taste perception mechanism of Pieris rapae's addiction to glucosinolates from the behavioral, cellular, and molecular levels
    .


    They found that the five representative glucosinolates in cabbage can significantly stimulate the larvae of Pieris rapae to eat


    So, how do the receptors expressed on these taste-receiving cells tune the different types of glucosinolates? They then used transcriptome sequencing and fluorescence quantitative PCR to screen two highly expressed bitter taste receptor genes, PrapGr28 and PrapGr15 , from the taste organs of female adults of Pieris rapae, and speculated that they might be involved in the perception of glucosinolates
    .


    Further through Xenopus oocytes and double-electrode voltage clamp experiments, it was found that only the oocytes expressing the bitter taste receptor gene PrapGr28 were sensitive to myrosin stimulation


    Postdoctoral researcher Yang Jun from the Institute of Zoology, Chinese Academy of Sciences is the first author of the paper, researcher Wang Chenzhu is the corresponding author, Guo Hao, Jiang Nanji, Tang Rui, Li Guocheng, Huang Lingqiao, and Professor Joop JA van Loon from Wageningen University Participate in the research of the subject
    .


    The achievement of this achievement has benefited from the combination of traditional electrophysiological techniques with modern omics and molecular biology methods, and provides strong evidence for the theory of co-evolution of insects and plants


    At the same time, PLoS Genetics published a forward-looking review of the research by Professors Noah K.
    Whiteman and Julianne N.
    Peláez of the University of California, Berkeley, and believed that the research was in the field of neurobehavioral and sensory ecology of herbivorous insects.
    The important progress made is a solid step towards fully revealing the taste molecular basis of the addiction of glucosinolates by cruciferous insects and the secret of the complex taste system of herbivorous insects
    .

      Link to the paper: https://doi.
    org/10.
    1371/journal.
    pgen.
    1009527 ; Link to the review article: https://journals.
    plos.
    org/plosgenetics/article?id=10.
    1371/journal.
    pgen.
    1009616

    Figure 1 The taste perception mechanism of the adult and larvae of Pieris rapae recognizing glucosinolate, the secondary substance of the host plant
    .


    Picture quoted from Whiteman and Peláez (2021)


    Figure 2 Identification of taste receptors of Pieris rapae that sense myrosin


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