Deng Hongkui's research group established a culture system for human potential expanded pluripotent stem cells (EPS cells)

2021 Nian 5 Yue 21 days, life Joint Center for Science, College of Life Sciences Denghong Kui research group in Nature Communications magazine published a paper entitled & ldquo; Chemically Defined and Xeno-as Free Culture for condition Condition for Human Extended Pluripotent Stem Cells & rdquo; research papers in A culture system of human potential expanded pluripotent stem cells ( EPS cells) with clear ingredients and no heterologous components was established in vitro , and a reprogramming induction method for inducing somatic cells into heterologous human EPS cells was established by using this system

How to establish high-quality pluripotent stem cells has always been the core issue of stem cell research

In order to solve this problem, Deng Hongkui's research group conducted research on the cytokines and extracellular matrix secreted by the feeder layer, and established a culture system ( xeno-free EPS culture condition , Fig.

Figure 1 : Establishment of a human EPS cell culture system with definite ingredients and no heterologous sources

Deng Hongkui's research group further systematically studied the extra-embryonic development potential of human EPS cells without heterologous culture.

Figure 2 : Extra- embryonic development potential of xeno-free hEPS cells

The culture system eliminates the influence of the uncertain components of feeder cells, improves the stability of human EPS cell culture , and provides conditions for promoting the wide application of human EPS cells in differentiation, disease models and developmental biology

The Joint Center for Life Sciences, Professor Deng Hongkui of Peking University School of Life Sciences, Peking University Stem Cell Research Center Researcher Xu Jun and Professor Bai Yun are the co-corresponding authors of the paper.

Original link: https:// class="MsoNormal" >references

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