Deng Hongyu's research group and collaborators revealed that VMP1 and TMEM41B are DMV formation during betacoronavirus infection...

The betacoronavirus family belongs to positive-sense single-stranded RNA viruses, including SARS-CoV-2, SARS-CoV, and MERS-Co.

On May 11, 2022, Deng Hongyu's research group from the Institute of Biophysics, Chinese Academy of Sciences and Zhao Yan's research group from the School of Life Sciences, Southern University of Science and Technology published a paper entitled "VMP1 and TMEM41B are essential for DMV formation during β" in " Journal of Cell Biology " -coronavirus infection" research paper, revealing that the endoplasmic reticulum-localized autophagy proteins VMP1 and TMEM41B are essential host factors during betacoronavirus infection and play important roles in different steps of DMV formatio.

Endoplasmic reticulum-localized VMP1 and TMEM41B are key proteins in autophagy and lipid metabolism processe.

Previous studies have found that exogenously expressed β-coronavirus proteins nsp3 and nsp4 in mammalian cells can cause the narrowing of the endoplasmic reticulum lumen through interaction, and further bend to form DMV-like double-membrane vesicles, which provides a basis for studying the mechanism of DMV formatio.

The researchers performed a genome-wide RNAi screen in .

Fi.

Taken together, this study shows that the nonstructural proteins nsp3 and nsp4 of SARS-CoV-2 are located on both sides of the DMV membrane, respectively, and this unique distribution is important for the formation of DMV vesicles in the narrow endoplasmic reticulum bendin.

Assistant Professor Zhao Yan, School of Life Sciences, Southern University of Science and Technology, and Researcher Deng Hongyu, Institute of Biophysics, Chinese Academy of Sciences are the corresponding authors of this pape.

After the work was published, D.

Article link:

https://rupres.

(Contributed by Deng Hongyu Research Group)