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Design of the Hairpin Ribozyme for Targeting Specific RNA Sequences

 Last Update: 2021-03-02
 Source: Internet
 Author: User

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The hairpin ribozyme has been developed and designed to target and cleave efficiently heterologous RNA sequences in a
trans
-reaction (
1
–
3
). Heterologous RNA cleavage has been carried out, targeting rules defined, systems identified, and very effective in vivo downregulation of gene expression obtained using these design rules (
4
,
5
). This chapter describes the rules and steps necessary for designing the hairpin ribozyme to cleave specific target sequences efficiently.

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