Detailed description of rapid detection kit for influenza virus

According to the World Health Organization, the United States, Mexico and other countries have recently seen human infection with swine flu (A / H1N1) As of April 28, there have been 1995 suspected cases of swine flu in Mexico, including 26 confirmed cases and 149 deaths There are more than 100 suspected cases in the United States, 40 of which have been confirmed The World Health Organization announced in Geneva on the evening of the 27th that it would raise the warning level of the influenza pandemic from the current level 3 to level 4, which means that the epidemic can spread among people and cause "mass" outbreaks At present, the epidemic is still expanding, which is likely to cause a global pandemic Swine influenza is an acute respiratory infectious disease caused by swine influenza virus, which has been outbreak many times in history, but rarely infects people In this case, the patient was infected with influenza A virus strain with H1N1 subtype This virus has been confirmed to be transmitted by human beings After human infection, there will be high fever, low spirits, anorexia, cough and other symptoms Sneezing, coughing and physical contact could all cause the new swine flu virus to spread among people, scientists said The flu is highly contagious and early diagnosis can prevent its spread Therefore, rapid detection of influenza virus plays a decisive role in epidemic control The genome of influenza A virus consists of eight gene fragments which encode 10 to 11 proteins Among them, the surface glycoprotein of the virus has unique antigen activity and nucleic acid sequence, which is mainly used for subtype classification of influenza virus, including 1-16ha and 1-9na According to the difference of HA and Na, name influenza virus, such as H1N1, h3h2, etc Surface glycoproteins often mutate, so virus subtypes often change The core protein (NP) of the virus is compiled from highly conserved gene fragments and has high stability Chen Gongxiang, professor and doctoral supervisor of Sun Yat sen University, academic leader of clinical laboratory and diagnosis discipline of Sun Yat sen University Medical College, and Guangzhou Jianlun Biotechnology Co., Ltd jointly developed and produced a type A influenza virus detection kit, which is designed according to the virus core protein, and can quickly detect a type of influenza virus without failure due to the variation of virus subtypes Wuhan Virus Research Institute of Chinese Academy of Sciences and other domestic and foreign institutions have conducted clinical trials The results show that the company's rapid detection kit for influenza A virus can quickly detect different subtypes of influenza A virus in different hosts (poultry, migratory birds and mammals) In the experiment, there are two strains of swine influenza virus H1N1 The company's type a detection reagent can accurately detect them and give the results within 15 minutes The results of this experiment provide a basis for the rapid diagnosis of influenza virus in this epidemic The product has obtained the approval certificate issued by the State Food and drug administration This product based on monoclonal antibody and colloidal gold technology provides a reliable and rapid screening method for doctors and port workers The following is the related test contents of influenza A virus detection kit of Guangzhou Jianlun Biotechnology Co., Ltd (1) the test results of foreign well-known detection institutions show that the influenza A virus detection kit of Guangzhou Jianlun Biotechnology Co., Ltd can react with each strain of influenza A virus 1 A total of 19 strains of influenza A virus were used for trial The culture solution of influenza A virus was prepared into 3.5 × 105tcid * 50 / virus solution for each test, and 100 μ l was dropped at the sample adding place of influenza a reagent 15 minutes visual inspection results The test results of each virus strain are shown in table 1:2 The confirmed virus strains that have reacted with the monoclonal antibody used by influenza a reagent of Jianlun Biotechnology Co., Ltd are shown in Table 2 (2) the test results of Wuhan Institute of viruses, Chinese Academy of sciences are as follows: 1 According to the instructions, the method of influenza a detection reagent can accurately reflect the positive results of influenza A virus of different subtypes from different hosts (poultry, migratory birds and mammals) 2 For different subtypes of influenza A virus, when diluted 5-1000 times with virus culture medium, some of the test results of influenza A virus strains can show positive bands visible to the naked eye, and the results show that the kit has high sensitivity in detecting influenza A virus See Table 3 3 below 15 strains of adenovirus, ecovirus, Coxsackie B virus, human cytomegalovirus, rubella virus, respiratory syncytial virus, herpes simplex virus and Chlamydia Parakeet were detected by this kit Cell virus culture solution was used as the test sample, and the results were negative The results showed that the kit had no cross reaction with non influenza virus, and had good specificity The test results of Wuhan Virus Research Institute of Chinese Academy of Sciences and well-known foreign testing institutions show that the influenza a kit produced by Guangzhou Jianlun Biotechnology Co., Ltd can detect all strains of influenza A virus If influenza A is found positive, isolation is recommended first, then antiviral drugs are given, and samples are sent to influenza monitoring center for further confirmation of swine influenza subtypes For influenza A, no matter its subtype is swine flu virus, avian influenza virus or other subtypes, the targeted drugs are the same Giving the right drugs in the early stage of the disease can gain valuable time for the life of patients.