Detection of Digoxigenin-Labeled DNA Probes Hybridized to Plant Chromosomes In Situ
-
Last Update: 2020-11-24
-
Source: Internet
-
Author: User
Search more information of high quality chemicals, good prices and reliable suppliers, visit
www.echemi.com
Digoxigenin is widely used as a nonradioactive label for
in situ
hybridization to locate
DNA
sequences along chromosomes in plants (e.g.,
1
–
3
) and animals. In many cases, its use is similar to biotin (chapter 25 , or e.g.,
4
), but digoxigenin labels may give lower unspecific background signal and therefore enable more efficient detection of short or low copy sequences. Digoxigenin is usually incorporated into the DNA enzymatically by nick translation, random priming, polymerase chain reaction (
PCR
), or end labeling using the modified nucleotide digoxigenin-11-dUTP (
see
chapter 10 and chapter 11 ).
This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only.
This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of
the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed
description of the concern or complaint, to
service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content
will be removed immediately.