Detection of Sequence-Specific Protein-DNA Interactions by the DNA-Footprinting Technique
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Last Update: 2021-01-05
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Source: Internet
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Author: User
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The initiation of transcription of eukaryotic genes by RNA polymerases is controlled by complex interactions between nonhistone proteins and specific regulatory
DNA
sequences (promoters and enhancers) (
1
). In order to characterize and purify such transacting protein factors, a sensitive and accurate assay for sequence-specific DNA-binding proteins is the DNA-footprinting technique, which can be used to analyze the interaction of a complex mixture of proteins with a gene regulatory sequence(s) that is known to be important for the expression of that gene.
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