Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes
-
Last Update: 2021-02-28
-
Source: Internet
-
Author: User
Search more information of high quality chemicals, good prices and reliable suppliers, visit
www.echemi.com
The characterization of specific
DNA
sequences by means of hybridization techniques is of great relevance in the development of molecular biology. Because of its simplicity, the method described by Southern in 1975 (
1
) is widely used for such purposes. This method, also known as the Southern blot, is based on the hybridization of specific probes to DNA fragments immobilized onto a membrane filter after their separation by agarose gel electrophoresis. The identification of specific DNA fragments is possible since their relative positions are maintained during the transfer from the gel to the filter.
This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only.
This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of
the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed
description of the concern or complaint, to
service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content
will be removed immediately.