Detection of Verotoxin Genes VT 1 and VT 2 in Escherichia coli O157:H7 in Minced Beef Using Immunocapture and Real-Time PCR
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Last Update: 2021-02-18
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Source: Internet
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Author: User
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Escherichia coli
O157:H7 is a highly virulent pathogen that causes severe food poisoning in humans. Many outbreaks involving this pathogen have been linked to minced beef. A protocol is presented here to detect
E. coli
O157:H7 in minced beef. The method consists of an enrichment step in modified tryptone soya broth, followed by immunomagnetic separation and extraction of deoxyribonucleic acid. Real-time polymearse chain reaction, using hybridization probes, is used to detect the verotoxin genes 1 and 2 found in
E. coli
O157:H7. The assay has a detection limit of log
10
3.5/mL
E. coli
O157:H7 cells in minced beef.
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