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Determination method of macrolides and lincosamide drug residues-immunoassay method

 Last Update: 2021-09-20
 Source: Internet
 Author: User

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(7) Immunoanalysis (IA)

Immunoassay is an analysis technique based on the specific and reversible binding reaction of antigen and antibody.
It is suitable for the separation or detection of trace components in complex matrices.
It was gradually applied to drug residue analysis in the 1980s
.

Enzyme linked immunosorbent assay (ELISA), fluoroimmunoassay (FIA) and immunochromatographic assay (ICA) are mainly used in the immunoassay of MALs and Lincosamide drug residues

Silverlight et al.
reported an ELISA assay for Tilmicosin
.

Select desmycocin (tylosinB DES), which is related to the structure of Tilmicosin, to synthesize haptens and artificial antigens that mimic Tilmicosin.

Ye et al.
established an indirect competitive FIA method to detect sulfamethazine , tylosin and streptomycin residues in milk
.

The hapten linked to bovine serum albumin (BSA) is immobilized on a glass slide.

Le et al.
developed an ICA method for the detection of tylosin and tilmicosin residues in muscle, liver, fish and eggs
.

This method is based on the principle of competition and can effectively improve its sensitivity by scanning the optimal amount of nano-gold-labeled monoclonal antibodies

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