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(6) Self-ordered ring fluorescence microscopic imaging technique (self-ordered ring fluorescence microscopic imaging technique) The self-assembled ring (SOR) fluorescence microscopic imaging technique uses an inverted fluorescence microscope to deposit an analyte on the ring line.
The charge-coupled element camera system is used as a microscope image acquisition technology to collect images, convert the image signals into electrical signals and input them into a computer for data reading and image processing, and then apply software to analyze the relevant parameters of the obtained image reading
.
It has the advantages of low background interference, low sample consumption, simplicity, speed and no pollution to the environment
(7) Biosensor method (biosensor)
1) Molecularly imprinted sensor
When the template molecule (imprinting molecule) contacts the polymer monomer, multiple points of action will be formed.
This effect will be remembered through the polymerization process.
When the template molecule is removed, the polymer will form a spatial configuration with the template molecule.
Matching holes with multiple points of action, such holes will have selective recognition characteristics for template molecules and their analogs
.
Xuan-Anh et al.
2) Surface plasmon resonance sensor
Surface Plasmon Resonance (SPR) technology has high sensitivity, simple operation, real-time monitoring of the dynamic process of the reaction, only simple pretreatment of the sample, no labeling, no need to purify various biological components, and low sample consumption , The detection time is short, and it has been widely used in various research fields
.
Marchesini et al.