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Determination method of steroid anabolic hormone drug residue analysis technology (2)

 Last Update: 2021-09-20
 Source: Internet
 Author: User

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(3) High performance liquid chromatography (HPLC)

HPLC is an efficient and rapid separation and analysis technology developed in the 1970s, which can simultaneously determine the residues of multiple hormones
.

Compared with GC, HPLC is not limited by sample volatility and thermal stability.

1) UVD/DAD detection

Yu Qian established an HPLC method for the simultaneous detection of four ASs in livestock and poultry meat, including estriol , estradiol , ethinyl estradiol and estrone
.

The chromatographic separation adopts Eclipse XDB C ₁₈ (4.

Wu Qilu et al
.

studied the residual detection methods of 4 ASs in eggs, including estradiol, estrone, estriol and testosterone .

Koole et al.
reported a HPLC multi-residue detection method for ASs in cow urine
.

Using HypersilOODS (150mm×4.

2) FLD detection

Dong et al.
established an HPLC-FLD method for the detection of estradiol, estriol, 4-hydroxyestradiol and 2-methoxyestradiol in fish and shrimp
.

The sample was extracted with acetonitrile, purified by MSPD, and determined by HPLC-FLD

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