Determination method of sulfa drug residues (5)

(2) Gas chromatography (GC)

In the 1990s, GC has been widely used in the detection of drug residues in food
.

In the early analysis of SAs residues, the GC method was widely used.

1) Electron Capture Detector (ECD)

Due to the high sensitivity of ECD, many impurities have an impact on it.
The sample processing is more complicated, the purification requirements are high, and the professional requirements of operators are relatively strong.
The application in routine testing laboratories is limited, and the GC method is due to diazomethane.
The reagent is easy to explode and should not be used
.

After the HPLC method was maturely applied to the detection of SAs residues in animal-derived foods, the GC-ECD method was gradually eliminated

The standard method for the detection of SAs residues formulated in China in the early days is the GC method
.

The inspection and quarantine industry standard SN0221-9310 stipulates the determination method of sulfamethoxine and sulfaquinoxaline residues in chicken

2) Mass Detector (MSD)

Gas chromatography-mass spectrometry (GC-MS) is also one of the confirmation methods in SAs residue monitoring
.

The GC-MS method not only has high sensitivity, but also provides structural information of the analyte.

Xu Xiaomei et al.
established a method for the determination of sulfamethazine in pig tissues by GC-MS
.

The sample was extracted with chloroform-acetone, purified with silica gel and SCX ion exchange SPE column, and then purified with 0.

(3) Capillary electrophoresis (capillary electrophoresis, CE)

CE or High Performance Capillary Electrophoresis (HPCE) is a new type of liquid phase separation analysis technology that uses capillary as the separation channel and high-voltage direct current electric field as the driving force.
It has developed rapidly in recent years and has been widely used
.

Compared with GC and LC, CE has high resolution, fast speed, small injection volume, low organic solvent consumption, and low sample pretreatment requirements.

Ackermans et al.
used capillary zone electrophoresis analysis technology to determine 16 SAs residues in pork samples
.

The sample was homogeneously extracted with acetonitrile, and the supernatant after centrifugation was directly subjected to capillary zone electrophoresis analysis.

(4) Thin layer chromatography (TLC)

With the development of modern instrumental analysis technology, the application of TLC technology in residue analysis is gradually reduced due to factors such as operation and sensitivity
.

Sherma et al.
used TLC method to detect ST in honey
.

The extract was purified with an alumina-anion exchange SPE column, developed by Bratton-Marshall diazotization coupling reagent, and separated and detected by quantitative silica gel TLC