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6.
1.
4.
2 Determination method
There are many types of MALs and similar structures.
The safe use and residual detection of these drugs have always been the focus and research focus of various countries
.
Lincosamide drugs are often used simultaneously with MALs, so simultaneous multi-residue analysis is required
.
(1) Microbiological analysis
The microbiological method is a classic method for determining the content of MALs
.
Wang Min et al.
used the principle of microbial growth inhibition to qualitatively detect six MALs residues
(2) Thin layer chromatography (thinlayerchromatography, TLC)
The TLC method uses a thin-layer plate to separate the analyte in the sample extract, and then measure the change in absorption light between the sample and the standard sample or use the emission light thin-layer scanning method to determine the content of the analyte
.
Ramirez et al.
used a high-performance thin-layer chromatography (HPTLC)-bioautography method for the multi-residue detection of erythromycin and other drugs in milk
(3) Capillary electrophoresis (capillaryelectrophoresis, CE)
As one of the separation technologies, CE has a high separation efficiency, can analyze multiple components to be tested at the same time, and requires less time, and can be accurately quantified
.
However, there are some disadvantages.
For example, the sensitivity is often insufficient for samples with a small loading amount
(4) Gas chromatography (gaschromatography, GC)
The molecular weights of MALs and Lincosamide drugs are relatively large, and the structure contains more polar groups.
Direct GC analysis is not acceptable.
GC analysis can only be performed after derivatization
.
Luo et al.
established a GC detection method for lincomycin in salmon tissue
.
The lincomycin in salmon tissue is extracted with phosphate buffer solution, purified by C18 solid phase extraction, and then derivatized with N,O-bis(trimethylsilyl) trifluoroacetamide as a derivatization reagent to form a trimethyl The base silyl group is analyzed by GC and detected by a nitrogen-phosphorus detector (NPD)
.
Related Links: Pretreatment method of macrolide and lincosamide drug residue analysis technology-purification method
