echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Chemicals Industry > Chemical Technology > Determination of 6 kinds of penicillin residues in honey

    Determination of 6 kinds of penicillin residues in honey

    • Last Update: 2021-09-20
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

    5.
    2.
    4.
    6 Determination

    (1) Liquid chromatography conditions

    Chromatographic column: SunFire TM C 18 3.
    5 um, 150 mm×2.
    1 mm (inner diameter) or equivalent; see Table 5-26 for mobile phase and flow rate; column temperature: 30°C; injection volume: 20 μL
    .

    Table 5-26 Liquid chromatography gradient elution conditions

    (2) Mass spectrometry conditions

    Ion source: Electrospray ion source (ESI); Scan mode: Positive ion scan; Detection mode: Multi-reaction monitoring: Electrospray voltage: 5500V; Atomizing gas pressure: 0.
    055 MPa; Curtain gas pressure: 0.
    079 MPa; Auxiliary gas flow rate: 6 L/min; ion source temperature: 400℃; qualitative ion pair, quantitative ion pair and declustering voltage (DP), focus voltage (FP), collision energy (CE) and collision cell exit voltage (CXP) are shown in Table 5- 27
    .

    Table 5-27 Qualitative ion pair, quantitative ion pair, declustering voltage, focusing voltage, collision gas energy and collision cell exit voltage of 6 penicillins

    (3) Qualitative determination

    Under the same experimental conditions, if the test substance in the sample has the same retention time as the standard substance tested at the same time, and the selected two pairs of ion abundance ratios are consistent, it can be determined that the residue is present in the sample
    .

    (4) Quantitative determination

    Under the best working conditions of the instrument, the matrix mixed standard solutions prepared by penicillin G, penicillin V, naphthalene penicillin , oxacillin , o-chloropenicillin , and dicloxacillin standard stock solutions were used to inject samples respectively with the standard working solution concentration It is the abscissa and the peak area is the ordinate to draw a standard working curve
    .
    Use the standard working curve to quantify the sample.


    The response values ​​of penicillin G, penicillin V, ethoxynene penicillin, oxacillin, o-cloxacillin, and dicloxacillin in the sample solution should all be within the linear range determined by the instrument


    Under the above chromatographic conditions, the total ion current diagrams of penicillin G, penicillin V, naphthalene penicillin, oxacillin, o-cloxacillin, and dicloxacillin are shown in Figure 5-7, and the retention time and ion abundance ratio are shown in Table 5- 28


    Table 5-28 Retention time and ion abundance ratio of 6 penicillins

    Figure 5-7 Total ion current diagram of 6 penicillin standard substances

    Related Links: Determination of 6 Penicillin Residues in Honey Application Scope and Method Principles of Liquid Chromatography-Tandem Mass Spectrometry

     

     

    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.