Determination of 9 kinds of penicillin residues in puffer fish and eels-selection of analytical conditions

5.
2.
3.
7 Selection of analysis conditions

(1) Selection of extraction solution

In the study of this method, according to the characteristics of the puffer fish and eel samples and the properties of the tested objects, acetonitrile was used to deproteinize, and acetonitrile was used as the extraction solution to eliminate the interference of the matrix on the 9 penicillins
.
The extraction recovery rate of 9 penicillins in puffer fish and eel with acetonitrile was completed respectively .

Table 5-21 Effect of acetonitrile + ammonia water extraction of puffer fish samples on the recovery rate of 9 penicillins

Table 5-21 Effect of acetonitrile + ammonia water extraction of puffer fish samples on the recovery rate of 9 penicillins

(2) Selection of liquid chromatography analysis column

The chromatographic behaviors of 9 penicillins were compared using uBondapak C ₁₈ 300mm×4.
0mm chromatographic column and ZORBA×SB-C ₁₈ 3.
5um150mm×2.
1mm chromatographic column
.
Experiments have found that the sensitivity of these two chromatographic columns can meet the requirements.