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    Home > Chemicals Industry > Chemical Technology > Determination of 9 Penicillin Residues in Livestock and Poultry Meat——Linear Range and Lower Limit of Determination

    Determination of 9 Penicillin Residues in Livestock and Poultry Meat——Linear Range and Lower Limit of Determination

    • Last Update: 2021-09-20
    • Source: Internet
    • Author: User
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    5.
    2.
    1.
    8 Linearity range and lower limit of determination

    Formulation containing piperacillin , penicillin G, penicillin V, oxacillin , nafcillin at a concentration of 1.
    0ng / mL, 5.
    0ng / mL, 10ng / mL, 50ng / mL, 100ng / mL, 200ng / mL , respectively; Amoxicillin Sample matrix mixed standard solutions with concentrations of 2.
    0 ng/mL, 10 ng/mL, 20 ng/mL, 100 ng/mL, 200 ng/mL, and 400 ng/mL of cloxacillin , ampicillin, cloxacillin, and dicloxacillin.
    Measured under the conditions, the injection volume is 20uL, and the peak area is plotted against the concentration of each component in the standard solution.
    The absolute amounts of piperacillin, penicillin G, penicillin V, oxacillin, and nafcillin are in The range of 0.
    02~4ng; the absolute amount of amoxicillin, ampicillin, cloxacillin, and dicloxacillin are linear in the range of 0.
    04~8ng respectively, which meets the quantitative requirements.
    The linear equations and correction factors are shown in Table 5-10
    .

    Table 5-10 Linear equations and correction factors of 9 penicillins

    Under the operating conditions of this method, when the added level of 9 penicillins was in the range of 0.
    25-20μg/kg (LOD-10LOD), the respective measured response values ​​were within the linear range of the instrument
    .


    At the same time, the additive concentration of nafcillin in the sample is 0.


    5.
    2.
    1.
    9 Method recovery and precision

    Use samples without penicillin to perform addition recovery and precision experiments.
    Add 0.
    25~20μg/kg (1 LOD~10 LOD) four different levels of 9 penicillin standards to the sample and shake them up to make the standard and the sample sufficient Absorb, then extract and purify according to this method, and measure with high performance liquid chromatography-tandem mass spectrometer.
    The recovery rate and precision are shown in Table 5-11
    .


    It can be seen from Table 5-11 that the recovery data of this method is between 73.


    Table 5-11 Experimental data of recovery rate of 9 penicillins added (n=10)


     

     

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