Determination of Berberine Hydrochloride in Guilin Watermelon Frost

Determination of Berberine Hydrochloride in Guilin Watermelon Frost

[Prescription] Watermelon Frost Calcined Borax Phellodendron Huanglianshan Dougen Shegan

Zhe Fritillaria, Indigo and Borneol Sapindus Fruit (Charcoal) Rhubarb, Scutellaria, Licorice and Menthol

[Content determination] Phellodendron amurense and Coptidis: According to the 2015 edition of "Chinese Pharmacopoeia" four parts of high performance liquid chromatography (General Principle 0512) determination

Chromatographic column: Reprosil C ₁₈ aq4.

Column temperature: 25℃

Flow rate: 1.

Instrument configuration: quaternary pump with vacuum deaerator (G1354A)

Autosampler (G1367A)

Column thermostat (G1316A)

UV detector (G1314A)

Instrument control and data processing: Agilent Chemstation

Preparation of reference substance solution: Take an appropriate amount of berberine hydrochloride reference substance, accurately weigh it, and add a mixed solution of hydrochloric acid-methanol (1:100) to make a solution containing 10ug per 1ml, and get it

Determination method: accurately draw 5ul each of the reference solution and the test solution, inject it into the liquid chromatograph, and measure, and it is obtained

Note: The mobile phase ratio is acetonitrile-0.

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