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    Home > Biochemistry News > Biotechnology News > Determination of blood glucose (glucose oxidase - peroxidase method)

    Determination of blood glucose (glucose oxidase - peroxidase method)

    • Last Update: 2020-10-22
    • Source: Internet
    • Author: User
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    "the sugar in the blood is mainly glucose, it is the form of sugar transport in the body.the absorbent value of the red quinone class ef with a wavelength of 505nm color was used, and

    < the glucose content in the specimen was calculated by comparing the > with the standard liquid absorbance value of the glucose.
    . < "> serum)

    add-in (mL)

    blank tube

    standard tube

    .

    the tube

    plasma (

    -

    <"top" width"88" >

    -

    10"L

    .

    glucose standard liquid (5.55mmol/L)

    -

    10"L

    -

    .

    distilled water

    .

    10"L

    -

    < "p style" text-align: left; ">-

    ase-phenol blend< >a href

    < "top" width "88" >.

    1.0mL

    .

    1.0mL

    The >1.0mL

    is mixed, 37 degrees C water bath insulation for 15 minutes, withhydrometer505nm color ratio, with a blank tube zero point, respectively, read out each tube absorbent.

    if the use of automaticbio-chemical analyzer determination, according to the instrument's procedural requirements.

    calculation:

    blood sugar mmol/L (measuring tube absorbance/standard tube absorbance) ×5.55 or blood sugar mg/dl (measuring tube absorbance/standard tube absorbance) ×100

    if blood sugar is above 22mmol/L, the serum (pulp) is diluted and then measured, the results are multiplied by dilution.

    reference normal value:

    serum (pulp) blood sugar 4.5 to 5.5mmol/L (70 to 110mg/dl)

    .
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