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"the sugar in the blood is mainly glucose, it is the form of sugar transport in the body.the absorbent value of the red quinone class ef with a wavelength of 505nm color was used, and
< the glucose content in the specimen was calculated by comparing the > with the standard liquid absorbance value of the glucose.add-in (mL) | blank tube | standard tube | . the tube |
plasma ( | < "> serum)
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-
10"L
glucose standard liquid (5.55mmol/L)
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10"L
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distilled water
10"L
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ase-phenol blend< >a href
1.0mL
.1.0mL
The >1.0mL
is mixed, 37 degrees C water bath insulation for 15 minutes, withhydrometer505nm color ratio, with a blank tube zero point, respectively, read out each tube absorbent.
if the use of automaticbio-chemical analyzer determination, according to the instrument's procedural requirements.
calculation:
blood sugar mmol/L (measuring tube absorbance/standard tube absorbance) ×5.55 or blood sugar mg/dl (measuring tube absorbance/standard tube absorbance) ×100
if blood sugar is above 22mmol/L, the serum (pulp) is diluted and then measured, the results are multiplied by dilution.
reference normal value:
serum (pulp) blood sugar 4.5 to 5.5mmol/L (70 to 110mg/dl).