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2 Determination method
CAPs residue analysis methods mainly include microbiological methods, immunoassays, thin-layer chromatography, capillary electrophoresis, chromatography and color-mass spectrometry
.
The immunological determination method established based on the antigen-antibody specific reaction has the advantages of high sensitivity, strong specificity, simple pretreatment, short analysis time, etc.
(1) Microbiological analysis (MA)
The main principle of the microbiological method is that if there are antibiotic residues in the sample, it will produce a zone of inhibition to the bacteria in the culture medium, and the result can be judged according to the existence and size of the zone of inhibition
.
This method is simple, low cost, but time-consuming, low sensitivity and specificity, easy to miss the detection, false positives may also occur, causing misjudgment, and it is only suitable for screening high residue samples
6) Immuno-PCR method (immuno polymerase chain reaction, IPCR)
IPCR is a micro-antigen detection technology established by using the specificity of the antigen-antibody reaction and the extremely high sensitivity of the PCR amplification reaction
.
Use a known DNA molecule to label an antibody as a probe, use this probe to react with the antigen to be tested, PCR amplify the DNA molecule attached to the antigen-antibody complex, electrophoresis qualitatively, according to the presence or absence of specific PCR products , To determine whether the antigen to be tested exists
Related links: Purification method of chloramphenicol residues (3)
