Determination of chloramphenicol, thiamphenicol and florfenicol residues in edible animal muscle, liver and aquatic products-linear range and lower limit of determination

4.
2.
2.
8 Linearity range and lower limit of determination

Mix 0μg/L, 0.
05ug/L, 0.
1μg/L, 0.
5μg/L, 1.
0μg/L, 2.
0μg/L and 5.
0μg/L of mixed standard working solution with water.
Each milliliter of the mixed standard working solution contains 3ng deuterated chloramphenicol
.

Table 4-11 Quantitative ion pairs, linear equations, and correlation coefficients of chloramphenicol, thiamphenicol and florfenicol

4.
2.
2.
9

Method recovery and precision

Supplemental recovery and precision experiments were carried out with chloramphenicol-free muscle, fish, shrimp and liver samples
.

Table 4-12 Addition recovery rate and precision experimental results (n=10)

It can be seen from Table 4-12 that the average recovery rate of chloramphenicol , thiamphenicol and florfenicol in muscle, liver, fish and shrimp meat is between 0.
1～4.
0ug/kg and 77.
3%～118.
0%.
, The relative standard deviation is 4.
0%～16.
4%
.

4.
2.
2.
10 Repeatability and reproducibility

Under repeatability test conditions, the absolute difference between the two independent test results obtained does not exceed the repeatability limit (r).
If the difference exceeds the repeatability limit (r), the test results should be discarded and the two individual tests should be completed again.
Determination
.

Table 4-13 The content range and repeatability and reproducibility equation of chloramphenicol, thiamphenicol and florfenicol (substrate is animal muscle)

Table 4-14 The content range and repeatability and reproducibility equation of chloramphenicol, thiamphenicol and florfenicol (substrate is animal liver)