Determination of Danshensu in Compound Danshen Dripping Pills

 Determination of Danshensu in Compound Danshen Dripping Pills

[Prescription] Danshen Panax Notoginseng Borneol

[Content determination] Danshensu was determined by high-performance liquid chromatography (General Principle 0512) according to the 2015 edition of "Chinese Pharmacopoeia"

Chromatographic conditions and system suitability test: Use Waters Acqulity UPLCTM HSS T3 (column length 100mm, inner diameter 2.

Table 19-21 Gradient elution conditions for determination of Danshensu content in Compound Danshen Dripping Pills by HPLC

Preparation of reference substance solution: Take an appropriate amount of Danshensu sodium reference substance, accurately weigh it, and add 75% methanol to make a solution containing 0.

Preparation of the test solution: Take 10 pills of this product, accurately weigh them, place them in a 10ml measuring bottle, add an appropriate amount of water, and ultrasonically treat (power 120W, frequency 40kHz) for 15 minutes to dissolve, let cool, add water to the mark, shake well, Filter, take the filtrate, and get it

Determination method: accurately draw 2~4ul each of the reference solution and the test solution, inject into the liquid chromatograph, and measure, and it is obtained

Each pill of this product contains Danshen based on Danshensu (C ₉ H ₁₀ O ₅ ), not less than 0.

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