Determination of linear range and detection limit of zearalenol, zearalenone, diethylstilbestrol, diethylstilbestrol and diethylstilbestrol residues in puffer fish, eels and grilled eels

9.

Formulated zeranol , corn rice gibberellic ketone , hexestrol , diethylstilbestrol , dienes female phenol standard concentrations were 2.

Table 9-16 Linear equation and correlation coefficient of the measured object

Since the tested substances are all banned veterinary drugs, the experiment uses 1/2 of the minimum detection limit (MRL) as the lower limit of detection, and 10 times the MRL is the upper limit of detection.

9.

Use samples of puffer fish and eels that do not contain zearalenol, zearalenone, diethylstilbestrol, diethylstilbestrol, and hexestrol for addition recovery and precision testing.

Table 9-17 The experimental data of the recovery rate of 5 kinds of hormones and metabolites in eel

Table 9-18 Experimental data on the recovery rate of five hormones and metabolites in puffer fish

9.

Under repeatability conditions, the absolute difference between the two independent test results obtained does not exceed the repeatability limit r, and under the reproducibility conditions, the absolute difference between the two independent test results obtained does not exceed the reproducibility limit R, fish tissue The content range and reproducibility equation of zearalenol, zearalenone, diethylstilbestrol, diethylstilbestrol and diethylstilbestrol are shown in Table 9-19

Table 9-19 Content range and repeatability and reproducibility equation

If the difference exceeds the repeatability limit r, the test results should be discarded and the determination of two single tests should be completed again