Determination of linear range and lower limit of 8 macrolides and lincosamide residues in royal jelly and royal jelly freeze-dried powder

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8 Linearity range and lower limit of determination

Prepare mixed standard solutions of different concentrations with the blank matrix solution, and measure under selected conditions.
The injection volume is 20μL.
The peak height is plotted against the concentration of each component in the standard solution
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The absolute amounts of lincomycin, erythromycin , tilmicosin , tylosin , spiramycin , clindamycin , kitasamycin and josamycin are linear in the range of 0.

Table 6-25 Linear equations and correlation coefficients of 8 antibiotics

Under the operating conditions of this method, when the added level of 8 antibiotics is in the range of 2.
0-50μg/kg, the response value is within the linear range of the instrument, and the signal-to-noise ratio S/N is greater than 5
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According to the sample volume represented by the final sample solution, the constant volume, the injection volume and the interference in the measurement, the royal jelly of this method is determined: lincomycin, erythromycin, tilmicosin, tylosin Lyophilized powder: lincomycin, erythromycin, clindamycin, tylosin and tylosin.

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9 Method recovery and precision

Use royal jelly samples without 8 kinds of antibiotics for addition recovery and precision experiments.
After adding 2.
0μg/kg, 4.
0μg/kg, 8.
0μg/kg, 20.
0μg/kg different concentration standards to the sample, shake it up to make the standard sample Fully absorb, and then perform extraction, purification, and determination according to this method.
The recovery rate and precision are shown in Table 6-26
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Experimental data shows that the recovery rate of this method is between 80.

Table 6-26 The recovery rate and precision experimental data of 8 kinds of antibiotics (n=10)

Related Links: Determination of Residues of 8 Macrolides and Lincosamides in Royal Jelly and Royal Jelly Lyophilized Powder