Determination of linear range and lower limit of carbachol, olaquindox and metabolite residues in liver and muscle of cattle and pigs

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2.
1.
8 Linearity range and lower limit of determination

Under the above-identified instrument detection conditions, take a series of standard solutions (n=8), take the peak area ratio of the analytical compound and the internal standard compound as the Y axis, and take the concentration ratio of the analytical compound and the internal standard compound as the X axis as the standard Curve, the results show that the concentration of the four analytical compounds has a good linear relationship with the peak area value (as shown in Table 15-5)
.

Table 15-5 The linear relationship between the concentration of each analytical compound and the peak area

Through sample addition and recovery experiments, it is true that the lower limit of determination of CBX, QCA, DCBX, and MQCA in animal tissues by this method is 0.
5 ug/kg, and 0.
5 ug/kg is added to pork, beef, pig liver, and beef liver that do not contain target analytes.
ug/kg
.

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2.
1.
9 Method recovery and precision

The recovery rate and precision test of this method used pork without CBX, DCBX, QCA and MQCA, pig liver, beef, and beef liver as the additive matrix, and set 0.
5 μg/kg, 1.
0 μg/kg, 2.
0 μg/kg , Four addition concentrations of 5.
0 μg/kg, each concentration was tested ten times, and the recovery rate of various analytes was measured as a summary list (see Table 15-6)
.

Table 15-6 Indoor recovery rate and precision results (n=10)

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2.
1.
10 Repeatability and reproducibility

Under repeatability test conditions, the absolute difference between the two independent test results obtained does not exceed the repeatability limit (r).
If the difference exceeds the repeatability limit (r), the test results should be discarded and the two individual tests should be completed again.
Determination
.

Table 15-7 Content range and repeatability and reproducibility equation