Determination of Magnolol and Honokiol in Xiangsha Yangwei Pills

Determination of Magnolol and Honokiol in Xiangsha Yangwei Pills

[Prescription] Woody 210g Amomum 210g Atractylodes 300g Chenpi 300g

Poria 300g Pinellia (prepared) 300g Vinegar incense 210g Citrus aurantium (stir-fried) 210g

Cardamom (shelled) 210g ginger magnolia root 210g patchouli 210g licorice 90g

[Content determination] According to the 2015 edition of "Chinese Pharmacopoeia" four parts high performance liquid chromatography (General Principle 0512) determination

Chromatographic conditions and system suitability test: octadecylsilane-bonded silica gel was used as filler; acetonitrile-water-glacial acetic acid (60:38:2) was used as mobile phase; detection wavelength was 294nm

Note: The mobile phase is methanol-water- glacial acetic acid (60:38:2); the injection volume is 5ul

Chromatographic column: Zorbax SB C ₁₈ , 4.

Column temperature: 25℃

Flow rate: 1.

Instrument configuration: quaternary pump with vacuum deaerator (G1354A)

Autosampler (G1367A)

Column thermostat (G1316A)

Diode array detector (G1315B)

Instrument control and data processing: Agilent Chemstation

Preparation of reference substance solution: take appropriate amount of magnolol reference substance and honokiol reference substance, accurately weigh them, and add methanol to make a mixed solution containing 0.

Preparation of the test solution: Take an appropriate amount of this product, grind it finely, take 2.

Determination method: accurately draw 10ul each of the reference solution and the test solution, inject it into the liquid chromatograph, and measure, and it is obtained