Determination of Oxytetracycline, Tetracycline and Chlortetracycline Residues in Livestock and Poultry Meat

This article introduces the high performance liquid chromatography method to determine the residues of oxytetracycline, tetracycline and chlortetracycline in livestock and poultry meat (according to GB/T5009.
116-2003)
.

1.
Principle

The sample is extracted, filtered by a microporous membrane, and injected directly, separated by reversed-phase chromatography, detected by ultraviolet detector, and quantified compared with the standard.
The peak sequence is oxytetracycline , tetracycline , and chlortetracycline
.

Calculate as follows:

In the formula, X——the antibiotic content in the sample, mg/kg

m ₁ ——The quality of the antibiotic measured in the sample solution, ug

m ₂ ——the mass of the sample, g

2.

Chromatographic column: 0DS-C18 (5um) 6.
2mm × 15cm; detection wavelength: 355nm; sensitivity: 0.
002 AUFS; column temperature: room temperature; flow rate: 1.
0 mL/min; injection volume: 10 uL; mobile phase: acetonitrile + 0.
01mol/ L sodium dihydrogen phosphate solution ( adjust pH 2.
5 with 30% nitric acid solution)=35+65, degas with ultrasonic for 10min before use
.

3.
Tips

This method is suitable for the determination of oxytetracycline, tetracycline, and chlortetracycline residues in various livestock and poultry meats
.