Determination of Polysaccharides in Mishitong Capsules

Determination of Polysaccharides in Mishitong Capsules

【Prescription】Talcum powder, dry extract of sable leaves

【Assay】polysaccharide

Preparation of reference substance solution: take anhydrous glucose reference substance, accurately weigh it, add water to dissolve it to make a solution containing 0.

Preparation of the test solution: Take the contents under the item of the difference in the amount of this product, grind it, take 2g, accurately weigh it, and place it in a Soxhlet extractor.

Preparation of total sugar test solution: accurately measure 5ml of test solution, add 5ml of 6mol/L hydrochloric acid solution, heat it in a boiling water bath for 30 minutes, then take it out; cool, add 1 drop of phenolphthalein indicator solution , use 6mol/L The sodium hydroxide solution was neutralized to reddish, quantitatively transferred to a 25ml measuring flask, and diluted to the mark, shaken, centrifuged, and the supernatant was taken and set aside

Preparation of reducing sugar test solution: accurately measure 5ml of test solution, put it in a 25ml measuring flask, add water to dilute to the mark, shake well, and get it

Determination method: Precisely measure 2ml each of the test solution, total sugar test solution and reducing sugar test solution, put them in a 25ml measuring flask, add 1.

This product contains molluscs polysaccharide based on anhydrous glucose (C ₆ H ₁₂ O ₆ ), not less than 4.

Determination of Polysaccharides in Ganbifu Capsules

This product is a capsule of Ganaderma applanaum (Pe rs.

Preparation of the test solution: Take the powder under the item of the difference in the content of this product, grind it, and accurately weigh out about 0.

Reducing sugar test solution: Precisely measure 6ml of the supernatant of the backup solution, place it in a 10ml measuring flask, add water to dilute to the mark, shake well, and get it

Determination method: Precisely measure 0.

(Formula 19-9) In the formula, A pair, A total, and A are the reference substance, total sugar, and reducing sugar test solution absorbance in turn; C pair is the concentration of the control solution (mg/ml); W is the sample amount (mg); P is the average grain weight (mg)

Note: The preparation of 3,5-dinitrosalicylic acid solution: Weigh crystalline powder 6.

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