Determination of Purity of Ursolic Acid—High Performance Liquid Chromatography (2)

3.

The recommended chromatographic column and typical chromatographic operating conditions are shown in Table 1.

Table 1 Chromatographic column and typical chromatographic operating conditions

3.

3.

Weigh 0.

3.

Weigh 0.

3.

According to the high performance liquid chromatography operating procedures, turn on the preheating, adjust the temperature and flow, and after the analysis conditions are reached and the baseline is stable, use the micro injection needle (for HPLC) to take the standard sample solution (3.

3.

The mass fraction w of ursolic acid, expressed in %, is calculated according to formula (1):

Where:

A ₁ ——The peak area of ​​ursolic acid in the sample solution;

A ₂ ——The peak area of ​​ursolic acid in standard sample solution;

m _s ——the value of the mass of the standard sample of ursolic acid in the standard sample solution, in grams (g);

m——The value of the mass of the sample, in grams (g)

w _s ——The mass fraction of ursolic acid in the standard sample of ursolic acid, %

4 Repeatability

Take the arithmetic average of the two parallel determination results as the determination result, and the absolute or relative difference between the two parallel determination results meets the requirements of the product standard

5 Test report

The test report should include the following:

a) Sample name, batch number, sampling location, sampling port period and time, etc.

b) Code of this standard;

c) Analysis results;

d) Any abnormal phenomena observed in the measurement and any details and explanations;

e) The name of the analyst and the date of analysis