Determination of relative correction factor

B.

By measuring the calibration standard sample composed of the main body with known mass ratio and the impurity components in the sample, the ratio of each impurity component to the main body's response value is its relative mass correction factor

B.

The reagents used to prepare the calibration standard samples should be chromatographically pure, and the mass fraction of the background samples should not be less than 99.

B.

Add an appropriate amount of the test component into a pre-weighed and sealable ground bottle, dilute it with a background sample, and shake it well: the added amount is weighed to the nearest 0.

Under the same chromatographic operating conditions as the measurement sample, the above-mentioned calibration standard sample and background sample were measured repeatedly 4 times, and the peak area of ​​each component was measured

B.

The correction factor fi of each component relative to the main body is calculated according to formula (B.

Where:

As——the value of the peak area of ​​the main body in the calibration standard sample, in square centimeters (cm³) or millivolt minutes (mV·min);

m _i ——the value of the mass of component i in the calibration standard sample, in grams (g);

A _i ——the value of the peak area of ​​component i in the calibration standard sample, in square centimeters (cm³) or millivolt minutes (mV·min);

A _{io ——the} value of the peak area of ​​component i in the background sample, in square centimeters (cm ² ) or millivolt minutes (mV·min);

m _s ——the value of the background sample amount in the calibration standard sample, in grams (g)

Note: When the unknown component is extracted, the relative correction of the neighboring component is due to calculation

B.

The relative correction factor should be actually measured, and should be checked regularly