Determination of Schisandrin A in Shenqi Wumaizi Tablets
【Prescription】Southern Schisandra 180g Codonopsis 60g Astragalus 120g Fried Jujube Seed 30g
[Content determination] According to the 2015 edition of "Chinese Pharmacopoeia" four parts high performance liquid chromatography (General Principle 0512) determination
Chromatographic conditions and system suitability test: octadecylsilane-bonded silica gel was used as filler; acetonitrile -water- acetic acid (64:35:1) was used as mobile phase; detection wavelength was 249nm
Number of theoretical plates Deoxyschizandrin peak should not be less than 4000
Figure 19-12 HPLC chart of Shenqi Wumeizi Tablets
Chromatographic column: Zorbax SB C 18 , 4.
6mm×250mm, 5um (883975-902)
Column temperature: 25℃
Flow rate: 1.
Instrument configuration: quaternary pump with vacuum deaerator (G1354A)
Column thermostat (G1316A)
UV detector (G1314A)
Instrument control and data processing: Agilent Chemstation
Preparation of reference substance solution: Take an appropriate amount of Schizandrin A reference substance, accurately weigh it, and add methanol to make a solution containing 60ug per 1ml, and get it
Preparation of the test solution: Take 20 tablets of this product, remove the sugar coating from the sugar-coated tablets, accurately weigh, grind, take about 1g, accurately weigh, place in a stoppered Erlenmeyer flask, accurately add 25ml of methanol, close stopper, and weigh Determine the weight, ultrasonically treat it (power 250W, frequency 50kHz) for 20 minutes, let it cool, and then weigh it.
Use methanol to make up the lost weight, shake well, centrifuge, and take the supernatant to get it
Determination method: accurately draw 10ul each of the reference solution and the test solution, inject it into the liquid chromatograph, and measure, and it is obtained
Each tablet of this product contains schisandrae schisandrae (C 24 H 32 O 6 ), not less than 0.
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