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Determination of sorbic acid and benzoic acid content in beverages (1)

 Last Update: 2021-07-14
 Source: Internet
 Author: User

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Sorbic acid and benzoic acid , as preservatives allowed by the country, are widely used in food and beverages
.

However, excessive use of the two is harmful to human health

There are three national standard methods for the determination of sorbic acid and benzoic acid in food: gas chromatography, high performance liquid chromatography, and thin layer chromatography
.

This training uses gas chromatography (according to the first method of GB/T5009.

1.
Purpose requirements

(1) Clarify the basic terms of gas chromatography; understand the main components of gas chromatograph, the role of each main part and the selection method of working conditions; understand the principles of gas chromatography
.

(2) Learn how to prepare and process samples
.

(3) Learn to use a gas chromatograph, master the method's operation process and points of attention
.

Understand routine maintenance and common troubleshooting methods, and clarify the influence of operating conditions on separation in gas chromatographic analysis

2.
Principles of training

After the sample is acidified, sorbic acid and benzoic acid are extracted with ether , and then separated and determined by a gas chromatograph with a hydrogen flame ionization detector, and compared with the standard series for quantitative determination .

Training supplies

(1) Gas chromatograph: with hydrogen flame ionization detector
.

(2) Ether : Does not contain peroxide
.

(3) Petroleum ether : boiling range 30～60℃
.

(4) Hydrochloric acid
.

(5) Anhydrous sodium sulfate
.

(6) Hydrochloric acid (1+1): Take 100 mL of hydrochloric acid and dilute to 200 mL with water
.

(7) Sodium chloride acid solution (40g/L): Add a small amount of hydrochloric acid (1+1) to the sodium chloride solution (40g/L) for acidification
.

(8) Standard solution of sorbic acid and benzoic acid: accurately weigh 0.
2000g each of sorbic acid and benzoic acid, place them in a 100mL volumetric flask, dissolve them with petroleum ether -diethyl ether (3+1) mixed solvent and dilute to the mark
.

This solution is equivalent to 2.

(9) Standard solution of sorbic acid and benzoic acid: draw an appropriate amount of standard solution of sorbic acid and benzoic acid, and dilute with petroleum ether-diethyl ether (3+1) mixed solvent to the equivalent of 50, 100, 150, 200, 250 ug per 1mL Sorbic acid or benzoic acid

Related link: Determination of preservatives

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