Determination of the content of active ingredients in traditional Chinese medicine (5)

6.

Sugar and its derivatives are widely distributed in nature, and they exist in both the animal kingdom and the plant kingdom

Polysaccharides refer to high polymers formed by dehydration of more than 10 molecules of monosaccharides

7.

The method for determining the content of total lignans can be chromotropic acid colorimetry, second derivative spectroscopy and column chromatography-colorimetry

The main methods for determining the content of monomeric lignans include spectrophotometry, thin layer chromatography, high performance liquid chromatography and so on

8.

Many Chinese herb coumarins, such component quantitative colorimetric methods, fluorescent spectrophotometry, spectrophotometry, thin layer chromatography, high performance liquid chromatography, gas chromatography and the like

The color reaction of coumarin components can be used to generate colored substances, which can be measured in the visible light region

Thin-layer chromatography is one of the commonly used methods for the determination of coumarins

9.

The determination of the content of organic acids is not obvious in the titration of aqueous solution, and non-aqueous titration method can be used

Aromatic acids and other acids having a UV absorption, high performance liquid chromatography can be used to determine the content, such as chlorogenic acid, gallic acid, cinnamic acid, Danshensu, ferulic acid and the like

Fatty acids such as malic acid , succinic acid , malonic acid , citric acid , and tartaric acid can be developed using pH indicators such as bromophenol blue and bromocresol green; terpenoids such as ursolic acid and oleanolic acid can Sulfuric acid ethanol, phosphomolybdic acid reagent, etc.

Spectrophotometry can be used to determine the content of organic acids by single-wavelength method, dual-wavelength method, and derivative spectrophotometry; it can also be determined after adding a color developing agent (for example, oleanolic acid can be color-developed with vanillin-glacial acetic acid).

Other compounds without UV absorption can be quantified by supercritical fluid chromatography, such as supercritical fluid chromatography to determine the content of oleanolic acid; high performance capillary electrophoresis can also be used, such as high performance capillary electrophoresis (conductivity detector) to determine citric acid , malic acid content; some of the compounds can be used to make the generated derivatization with chemical derivatives volatility, measured by gas chromatography, such as 17- linolenic acid, under basic conditions, with a boron trifluoride - methanol reagent generated 1-methyl linolenic acid, determined by gas chromatography; royal acid (10-hydroxy-2-decenoic acid) under the catalysis of boron trifluoride , methanol and 10-hydroxy-2-decenoic acid under certain conditions Methylation reaction produces methylated derivatives, which are determined by gas chromatography

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