Determination of Total Flavonoids in Xiaokechuan Syrup and Epimedium in Kangguzengsheng Capsules

Determination of Total Flavonoids in Xiaokechuan Syrup

【Prescription】Manshan Red 2000g

【Assay】Total Flavonoids

(1) Preparation of reference substance solution: Take an appropriate amount of rutin reference substance, accurately weigh it, and add 60% ethanol to make a solution containing 60ug of rutin per 1ml, and get it

(2) Preparation of standard curve: accurately measure 0.

(3) Determination method: accurately measure 2ml of this product, put it in a 50ml measuring flask, add 60% ethanol (total flavonoids extracted with dilute alcohol) to the mark, shake well, accurately measure 1ml, put it in a 10ml measuring flask, according to the standard The method under the curve preparation item starts with "adding 0.

Determination of Epimedium in Kangguzengsheng Capsules

[Prescription] Rehmannia glutinosa 175g Cistanche cistanche 117g dog ridge (salt) 117g Ligustrum lucidum (salt) 58g

Epimedium 117g Caulis spatholobi 117g Fried raisin 117g Drynaria 117g Achyranthes 117g

[Content determination] According to the 2015 edition of "Chinese Pharmacopoeia" four parts high performance liquid chromatography (General Principle 0512) determination

The chromatographic conditions and system suitability test use octadecylsilane-bonded silica gel as the filler; use acetonitrile-water (30:70) as the mobile phase; the detection wavelength is 270nm

Chromatographic column: Zorbax XDB C ₁₈ , 4.

Column temperature: 25℃

Flow rate: 1.

Instrument configuration: quaternary pump with vacuum deaerator (G1354A)

Autosampler (G1316A)

Column thermostat (G1316A)

UV detector (G1314A)

Instrument control and data processing: Agilent Chemstation

Preparation of reference substance solution: Take an appropriate amount of icariin reference substance, accurately weigh it, and add methanol to make a solution containing 20ug per 1ml, which is ready

Preparation of the test solution: Take the contents of the product under the item of difference in volume, mix and grind it, take about 1g, accurately weigh it, place it in a 100ml conical flask with a stopper, and accurately add dilute ethanol (with dilute Total flavonoids extracted by alcohol) 50ml, densely plugged, weighed, ultrasonically treated (power 250W, frequency 33kHz) for 1 hour, let cool, make up the lost weight with dilute ethanol , shake well, filter, take the subsequent filtrate, and get it

Determination method: accurately draw 20ul each of the reference solution and the test solution, inject into the liquid chromatograph, and measure, and it is obtained

Note: The mobile phase ratio is acetonitrile-water (25:75), and the injection volume is 3ul

Each capsule of this product contains epimedium as icariin (C ₃₃ H ₄₀ O ₁₅ ), not less than 0.