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Determination of total flavonol glycosides in ginkgo leaves
This product is made of ginkgo biloba extract after processing
[Assay] Total flavonol glycosides: determined according to the 2015 edition of "Chinese Pharmacopoeia" four parts high performance liquid chromatography (General Principle 0512)
The chromatographic conditions and system suitability test use octadecylsilane- bonded silica gel as filler; use methanol- 0.
Column temperature: 25℃
Flow rate: 1.
Instrument configuration: quaternary pump with vacuum deaerator (G1354A)
Autosampler (G1367A)
Column thermostat (G1316A)
UV detector (G1314A)
Instrument control and data processing: Agilent Chemstation
Preparation of reference solution: Take appropriate amounts of quercetin reference substance, kaempferin reference substance, and isorhamnetin reference substance, accurately weigh them, and add methanol to make a mixed solution containing 30ug, 30ug, and 20ug per 1ml as a control Product solution; or take 35mg of the Ginkgo biloba reference extract that has been labeled with the content of loretin, kaempferin , and isorhamnetin, accurately weighed, and add 25ml of a mixed solution of methanol- 25% hydrochloric acid solution (4:1), according to The preparation method of the test solution is to prepare the control extract solution by the same method starting from "heating and refluxing in a water bath for 30 minutes"
Preparation of the test solution: Take 10 tablets of this product, remove the coating, accurately weigh, and grind, take the powder equivalent to 19.
Determination method: accurately draw 10ul each of the reference solution (or reference extract solution) and the test solution, inject into the liquid chromatograph, determine, and calculate the contents of pepitin, kaempferin and isorhamnetin respectively, press The following formula is converted to the content of total flavonol glycosides
Total flavonol glycoside content = (quercetin content + kaempferin content + isorhamnetin content) × 2.
Note: The mobile phase ratio is methanol-0.
Related Links: Determination of Total Flavonoids in Xiaokechuan Syrup and Epimedium in Kangguzengsheng Capsules