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    Home > Chemicals Industry > Chemical Technology > Determination of ursolic acid in Dashanzha Pills and chlorogenic acid in Shuanghuanglian for injection (freeze-dried)

    Determination of ursolic acid in Dashanzha Pills and chlorogenic acid in Shuanghuanglian for injection (freeze-dried)

    • Last Update: 2022-06-13
    • Source: Internet
    • Author: User
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    Determination of Ursolic Acid in Dashanzha Pills

    [Content determination] Take the product under the weight difference item, cut into pieces, mix well, take about 3g, accurately weigh it, add 30ml of water, warm it in a 60℃ water bath to dissolve it, add 2g of diatomaceous earth, stir and filter After that, the residue was washed with 30ml of water, dried at 100°C, and placed in a Soxhlet extractor together with the filter paper.


    Each pill of this product contains hawthorn based on ursolic acid (C 30 H 48 O 3 ), not less than 7.


    Determination of chlorogenic acid in Shuanghuanglian for injection (lyophilized)

    [Content determination] Honeysuckle according to the 2015 edition of "Chinese Pharmacopoeia" four parts high performance liquid chromatography (General Principle 0512) determination



    Note: The injection volume is 5ul

    Chromatographic column: Zorbax SB C 18 , 4.


    Column temperature: 25℃

    Flow rate: 1.


    Instrument configuration: quaternary pump with vacuum deaerator (G1354A)

    Autosampler (G1367A)

    Column thermostat (G1316A)

    UV detector (G1314A)

    Instrument control and data processing: Agilent Chemstation

    Preparation of reference substance solution: Take an appropriate amount of chlorogenic acid reference substance, accurately weigh it, place it in a brown measuring flask, add water to make a solution containing 20ug per 1ml, and get it


    Preparation of the test solution: Take the contents of the product under the item of difference in the filling amount, mix well, take 60mg, and accurately weigh it


    Determination method: accurately draw 20ul each of the reference solution and the test solution, inject into the liquid chromatograph, and measure, and it is obtained


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