Determination of zearalenol, zearalenone, diethylstilbestrol, hexestrol, and diethylstilbestrol residues in puffer fish, eels and grilled eels

9.

(1) Liquid chromatography conditions

Column: ZORBAXEclipse SB-C ₈ , 3.

(2) Mass spectrometry conditions

Ionization method: electrospray ionization; scanning method: negative ion scanning; detection method: multiple reaction monitoring (MRM); ion source temperature: 350℃; atomizing gas pressure: 0.

Table 9-15 Qualitative ion pairs, quantitative ion pairs, collision energy and declustering voltage of 5 hormones and metabolites

(3) Qualitative determination

Choose one parent ion and two or more product ions for each tested component.

(4) Quantitative determination

Prepare a series of mixed matrix standard working solutions to inject samples separately and draw standard working curves

Figure 9-4 Multi-reaction monitoring (MRM) chromatogram of zearalenol, zearalenone, diethylstilbestrol, hexestrol, and diethylstilbestrol standard substances

9.

Optimization of mass spectrometry conditions: using a syringe pump to directly inject samples, and inject standard solutions of zearalenol, zearalenone, diethylstilbestrol, hexestrol, and diethylstilbestrol into the ion source of the tandem mass spectrometer at 5 μL/min.

The collision gas energy (CE) is optimized for the selected product ions for each analyte

Related links: Determination of zearalenol, zearalenone, diethylstilbestrol, hexestrol, and diethylstilbestrol residues in puffer fish, eels and grilled eels by liquid chromatography-tandem mass spectrometry