Determination of Zearillol Residues in Aquatic Products - Liquid Chromatography-Tandem Mass Spectrometry (3)

7 Result calculation

The residual amount of zearalenol in the sample is calculated according to formula (1), the blank value is deducted from the measurement result, and 3 decimal places are retaine.

Single point calibration:

Or blank addition standard curve calibration: from As=aXs+b, find a and they are substituted into formula (1.

where:

X——the residual amount of zearabin in the test sample, in micrograms per kilogram (μg/kg);

Xs——concentration of the corresponding zearatol in the blank sample, in micrograms per kilogram (μg/kg);

A——the peak area of ​​the corresponding zeara alcohol in the sample solution to be tested;

As——the peak area of ​​the corresponding zeara alcohol in the blank added sample solution;

ms——mass of blank added sample, in grams (g);

m—the mass of the sample to be tested, in grams (g.

8 Method Sensitivity, Accuracy and Precision

1 Sensitivity

The detection limits of the six zeara alcohol residues were all 50 μg/kg, and the quantification limits were all 00 μg/k.

2 Accuracy

When the concentration range of this method is 00 μg/kg~200 μg/kg, the recovery rate is 60%~120.

3 Precision

The relative standard deviation between batches of this method is less than 15.