Determination steps of zearalenol residues in animal-derived foods

9.

(1) Liquid chromatography conditions

Chromatographic column: C ₁₈ , 2um, 50mm×2.

Table 9-28 Mobile phase and gradient elution conditions

(2) Mass spectrometry conditions

Ionization method: electrospray ionization (ESI); ion source spray voltage (IS): 3500V; ion source temperature (TEM): 350°C; GasFlow: 11L/min; Nebulizer: 40psi; DeltaEMV(-): 600V; scanning method: Negative ion scanning; detection method: multiple reaction monitoring (MRM), monitoring conditions are shown in Table 9-29

Table 9-29 Multiple reaction monitoring conditions

(3) Qualitative determination

Choose one parent ion and two or more product ions for each component to be tested.

(4) Quantitative determination

In the best instrument: under working conditions, use the mixed matrix standard calibration solution to inject samples separately, take the peak area as the ordinate and the concentration of the mixed matrix standard calibration solution as the abscissa, draw a standard working curve, and use the standard working curve to quantify the sample , The response value of the analyte in the sample solution should be within the linear range determined by the instrument

Figure 9-5 Multiple reaction monitoring (MRM) chromatogram of β-zearalenol standard substance

Figure 9-6 Multiple reaction monitoring (MRM) chromatogram of zearalenol standard substance

Figure 9-7 Multiple reaction monitoring (MRM) chromatogram of zearalenone standard substance

Related Links: Determination of Zearalenol Residues in Food of Animal Origin by Liquid Chromatography-Tandem Mass Spectrometry