Diabetogia: The deposition of hyaluronic acid in the islet may precede and guide the islet immune cell immersion position

Large deposits of hyaluronic acid (HA) are characteristic of dominant type 1 diabetesWe looked at whether HA accumulation can be detected in the islet early in the onset of the disease and how this affects the development of isletitis and beta cell clustersresearchers examined islet HA staining and myitist in 15 cases of islet autoantibodies (aAb) positive non-diabetic organ donors and 14 aAb-controlled donor pancreatic tissues of the same ageIn DRLyp/Lyp rats,a spontaneous autoimmune diabetes model, from 40 days to the onset of diabetes, islet HA deposition dynamics, insulitis incidence and progression, and changes in cell quality were studiedobserved rich islet HA deposition in 3 mono-ireactors and 4 cases of double aAb-HAhigh pancreatic tissueIn the seven tissues, the area of HA staining was measured in mice with an area of 1000 to 240 m2 (average sEM) and was 4 times larger than the area in the aAb-control groupAAb-HA high tissue also has a greater incidence, is highly abundant HA (21% of these tissues in the mouse tissue containing the largest HA staining area of the (2000 m2) with less than 1% of the tissue from the aAb-control donor tissue)The amount of islet HA staining is related to the positive number of single or double strains, and the amount of HA staining in these tissues is related to the number of aAb (i.e., single Ab or double Ab-positive), but not with changes in the quality of HLA genotype or beta cellsIn 7 aAb-HA-high tissues, 3 from a single aAb-plus donor and 1 tissue from a double aAb-plus donor did not show any mouse immune cell immersion, indicating that ha's accumulation in aAb-plus donors was not related to isletitisThe high tissue of 3 aAb-HA islet itisis, of which 3 aAb-HA high tissuehas have the largest HA immersion area, and in these tissues, the mouse transplanted immune cells were positioned jointly with the most prominent HA sediments (i.e., HA immersion area of 2000 m2)In asymptomatic pre-DRLyp/Lyp rats aged 7-8 weeks, the accumulation of HA in mice was evident, with the mice's HA dying area measured at 2370 to 170 m2 (average SEM), which was three times higher in 6-week-old ratsThis initial mouse HA deposition did not occur at the same time as beta cell lossIsletitis was first detected in rats aged 9-10 weeks, with HA staining area of 4980 to 500 m2At this age, rats also showed a 44 percent reduction in beta cell massThe further expanded HA-positive region (average SEM: 7220?880 m2) was associated with invasive isletitisHA sediments are still abundant in the renal tubes of rats with destructive renal tubeitist, 85% of which are lost beta cells this study shows that HA deposits in islet son son son son seasbefore earlier than type 1 diabetes, earlier than islet itis, and suggests that HA may trigger islet immune cell immersion, promoting isletitis