Dipsaponin B

Dipsaponin B

Dipsacoside B

【Structural formula】

【Molecular formula and molecular weight】C ₅₃ H ₈₆ O ₂₂ ;1074.

[Source] The dried flower buds of Lonicera confuse DC

【Properties】White powder

This product is soluble in methanol , ethanol and the like

Melting point: 206～211℃

【Purity Check】

TLC

1.

Developing solvent chloroform - methanol - water (13:7:2) the lower layer liquid

Check 10% sulfuric acid ethanol solution, heat at 105℃ until the spots are clear, check under sunlight and ultraviolet light (365nm)

2.

Developing solvent ethyl acetate - ethanol - water (7:2: 0.

Check 10% sulfuric acid ethanol solution, heat at 105℃ until the spots are clear, check under sunlight and ultraviolet light (365nm)

High performance liquid chromatography

Chromatographic column Agilent TC C ₁₈ , 5um (4.

Mobile phase acetonitrile-0.

Detector ELSD (nitrogen flow rate 2.

【Structural identification】203

3411 (br, OH), 2940 (C—H), 1733 (COOR), 1645 (C=C), 1457, 1388, 1261, 1076-1029 (C—O), 913

ESI-MS m/z: 1097[M+Na] ⁺ , 773[M+Na-Gle-Glc] ⁺ , 627[M+Na-Glc-GlcRha] ⁺

¹ H-NMR (CDCl ₃ ) δ: 5.

¹³ C-NMR (CDCl ₃ ) δ: 39.
1(C-1), 26.
1(C-2), 81.
1(C-3), 43.
5(C-4), 48.
2(C-5), 18.
2(C-6 ), 32.
9(C-7), 40.
0(C-8), 47.
8(C-9), 36.
92(C-10), 23.
4(C-11), 122.
9(C-12), 144.
2(C-13) , 42.
2(C-14), 28.
3(C-15), 23.
7(C-16), 47.
1(C-17), 41.
7(C-18), 46.
3(C-19), 30.
8(C-20), 34.
0(C-21), 32.
6(C-22), 64.
1(C-23), 13.
7(C-24), 16.
2(C-25), 17.
6(C-26), 26.
2(C-27), 176.
5 (C-28), 33.
1(C-29), 23.
9(C-30), 104.
2(C-1'), 76.
0(C-2'), 74.
2(C-3'), 69.
8(C-4') ), 65.
2(C-5'), 101.
6(C-1"), 72.
3(C-2"), 72.
6(C3"), 74.
1(C-4"), 69.
0(C-5"), 18.
5( C-6"), 95.
7(C-1'"), 75.
2(C-2'"), 78.
4(C-3"'), 71.
0(C-4'"), 78.
0(C-5'") , 69.
5(C-6'"), 105.
2(C-1""), 73.
9(C-2""), 78.
7(C-3""), 71.
6(C-4""), 78.
4( C-5""), 62.
7(C-6"")
.

【Storage】Dry at low temperature
.

Related Links: Glycoside