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    Home > Biochemistry News > Biotechnology News > DNA Capillary Electrophoresis Using Block Copolymer as a New Separation Medium

    DNA Capillary Electrophoresis Using Block Copolymer as a New Separation Medium

    • Last Update: 2021-03-03
    • Source: Internet
    • Author: User
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    The development of automated
    DNA
    sequencing has drawn the attention of many research groups in recent years, especially for the high-throughput demand of the Human Genome Project (
    1
    ,
    2
    ). Automation of DNA sequencing based on standard electrophoresis is the most commonly accepted and used approach. Capillary electrophoresis (CE) using replaceable polymer solution as a rapid separation medium with high-resolution separations can be automated relatively easily when compared with slab-gel electrophoresis. The development of capillary array electrophoresis (CAE) has further enhanced the throughput of DNA sequencing. The application of replaceable polymer solutions over permanent crosslinked gels as a separation medium made CE the best conventional analytical method at this time (
    3
    )f because gels tend to be more difficult to handle and have limited column lifetime.
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