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Dna fragments of agarose gel electrophoresis.

 Last Update: 2020-10-30
 Source: Internet
 Author: User

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"content"

"center" . < p-align"center" > DNA/Hind III.. < td valign>< "top" >

unenzyme lysed particles

ase lysolytesomes

< "center" >10 sl (lr)

10 sl (-2r)

. < "top" >

6× point sample liquid

2 sl

7, mix the above three tubes of samples on the shockor, short centrifugation.

distinguishable Linear DNA fragment size (kb)

0.4

5 to 60

0.7

< > 10

1.0

0.4 to 6

1.5

0.2 to 4

< the p-align> 1.750.2 to 3

2.0

0.1 to 3.

agarose gel concentration

distinguished linear DNA fragment size (kb) 3, DNA molecule morphology: in the same concentration of agarose gel, ultra-helical DNA molecule migration rate is faster than linear DNA molecules, linear DNA molecules than open-loop DNA molecules.

4, current strength: gel voltage per centimeter does not exceed 5V, if the voltage is too high resolution will be reduced, only at low voltage, linear DNA molecule electrophoresis migration rate is directly related to the voltage used.
.

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Capillary Electrophoresis of DNA Fragments with Replaceable Low-Gelling Agarose Gels

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Dna fragments of agarose gel electrophoresis.

Related Articles

Capillary Electrophoresis of DNA Fragments with Replaceable Low-Gelling Agarose Gels