DNA probes mark the preparation of commonly used reagents.

(1) 10 × pan buffer: 200mmol/l Tris-HCl, pH7.4 containing 50mmol /L MgCl2;
(2) gap translation reaction termination fluid: 200mmol/L NaCl; 10mmol/l Tris-HCl, pH7.4; 11mmol/L
. EDTA
; 0.5%
SDS
.
(3) DNaseI.: Dry powdered DNaseI. (2000 to 3000u/mg) dissolved in 20mmol/l Tris-HCl, pH7.5 (1mg/ml), 10 sl, -20C for one year.
(4) 10×
. DNA
polymerase I. (Klenow fragment) buffer: 500mmol/l Tris-HCl, pH6.6; 100mmol/L Mg-Cl2; 10mmol/L
DTT
; 0.5mg BSA.
(5)10×
Kinase
Buffer: 500mmol/L Tris-HCl, pH7.4, 50mmol/L MgCl2; 20mmol/L DTT; 1.0mmol/L subseminide.
(6)10× random lead marker buffer; 500mmol/l Tris-HCl, pH6.6; 100mmol/L MgCl2; 10mmol/L β- base ethanol; 500μg/ml BSA.
(7)1× tail buffer: 100mmol/L potassium dithyl, pH7.0, 1mmol/l CoCl2 0.2mmol/L DTT.
(8) 1mol/L MgCl2:MgCl2 47.60g dissolved in 500ml water, 100ml split, autocultension, room temperature preservation.
(9) 0.25mol/L EDTA (Ph8.0): EDTA 52.02g dissolved in 400ml water, pH to 8.0, added water to 500ml, 100ml displodged, autocultension, room temperature preservation.
(10) 4mol/L sodium acetate: take 82g of aqueous sodium acetate dissolved in 200ml water, pH to 6.5 with acetic acid, add water to 250ml, autocultension, or 0.45 m membrane
filtration
, room temperature preservation.
(11) 10% SDS: 10g SDS (sodium tetranyl sulfate) dissolved in 50 ml of water, added water to 100 ml, stored at room temperature after loading.
(12)20×SSC: Take NaCl 175.3g; sodium citrate 88.2g; add water to 1000ml, tune pH to 7.0 with 10mol/l NaOH; autocultension, room temperature preservation.
(13) sterile water: 100ml deionized or double steamed water, divided, autocultension, room temperature preservation, only one week after opening the bottle.
(14)10× Kinase buffer: 500mmol/L Tris-HCl, pH7.4; 100mmol/L MgCl2; 50mmol/l DTT; 10mmol/L subseminide (not required).
(15) T4 polymer
nucleotides
kinase: 10u/l, stored in glycerin, -20 degrees C.
(16) TE buffer (Tris/EDTA): Tris, 10mmol/l pH7.4 (0.5ml 2mol/L storage), 1.0mmol/l ED-TA, pH8.0 (20 sl 0.5mol/L) storage, plus water to 100ml, room temperature.
(17) 2mol/L Tris-HCl pH7.4: Tris242.2g dissolved in 850ml, thickened HCl 75ml, stirring slowly to pH7.4, plus water to 1000ml.
(18) 1mol/L DTT (dithioneol): 3.0g DTT dissolved in 20 ml of water, divided, stored at -20 degrees C.
(19) 0.5mol/L EDTA (acetylene diacetate di sodium salt): Add 300ml water to the
beaver
, add 93.5g EDTA-Na2.2H2O, mix well, add 10mol/l NaOH to 8.0, add water to 500ml.
(20) 10mol/L NaOH:200g NaOH dissolved in 450ml water, mixed well, then added water to 500ml.
(21) 5mol/L NaCl: 292.25g NaCl, plus water to 1000ml.
(22) 1mol/L HCl: Plus 86.2 ml of hydrochloric acid to 913.8 ml of water.
(23) 1mol/L CaCl2:147g CaCl2:2H2O, dissolved in 1000ml water, autocultension, room temperature preservation.
.