DNA打点法（DNA Dot Blot）
-
Last Update: 2020-10-27
-
Source: Internet
-
Author: User
Search more information of high quality chemicals, good prices and reliable suppliers, visit
www.echemi.com
1) membrane treatment: put on clean
gloves
, take out the nitric acid fiber membrane, cut as needed into a certain size, a good amount of distance between points, marked with a soft pencil.
2)
. DNA
degeneration: the DNA solution in a 1×TE (pH8.0) buffer or distilled water, take a certain amount of DNA samples added to a small plastic tube, boil 10min in boiling water at 100 degrees C, quickly into ice water.
3) dot: use plastic or siliconized glass or microscopic sampler to take 1 to 5 μl denatured DNA, the dropper on the nitric acid fiber membrane, so that the DNA slowly sucked on the membrane, after sampling cool dry.
4) Fix: Clamp the cool-dried membrane into the
filter paper
, dry roast 2 to 3h at 80 degrees C, and then hybridize..
This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only.
This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of
the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed
description of the concern or complaint, to
service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content
will be removed immediately.
