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    Home > Biochemistry News > Biotechnology News > DNase I Footprinting of Small Molecule Binding Sites on DNA

    DNase I Footprinting of Small Molecule Binding Sites on DNA

    • Last Update: 2021-02-27
    • Source: Internet
    • Author: User
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    Nuclease footprinting techniques were initially developed to investigate protein-deoxyribonucleic acid (
    DNA
    ) interactions but these tools of molecular biology have also become instrumental for probing sequence-selective binding of small molecules to DNA. Here, the method is described and technical details are given for performing deoxyribonuclease (DNase) I footprinting with DNA-binding drugs. An example is presented where DNase I is used (as well as DNase II and micrococcal nuclease) to probe the patterns of sequence-selective recognition of DNA by the anticancer antibiotic actinomycin D. DNase I is a convenient endonuclease for detecting and locating the position of actinomycin-binding sites within GC-rich sequences.
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