【Document Express】Nature Communications explores the mystery of zygotic genes

Doubts from fertilized eggs

Have you ever wondered whether the expression of genes during fertilization is controlled by the father or the mother? Have you ever wondered when the zygote begins to express the zygotic gene, and how does this process begin?

The research team of Nicola Iovino from Max Planck Institute, Germany, recently published a study on Nature Communications, Histone variant H2A.

First, the author analyzed the zygotic transcripts in the ZGA period through GRO-seq and found 6249 key genes, and 5680 of them do not depend on Zelda for activation transcription; confirmed by ChIP-seq, ATAC-SEQ, MNase-seq The transcription initiation sites of these genes are abundantly enriched in H2A.

Subsequently, the researchers found by IP-MS and H2A.

Then, the researchers proved that in DomKD, the transcription level of H2A.

Finally, the researchers used MNase-seq and TAD analysis (using the insulation score of the three-dimensional structure to evaluate the integrity of the three-dimensional structure

Based on the above research content, the researchers believe that H2A.

In this study, it is not difficult to find that the author frequently used the ChIP (chromatin immunoprecipitation) method

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