Dot method.
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Last Update: 2020-10-27
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Source: Internet
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Author: User
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.sample method is similar to in-place synthesis technology in the design of polymers. The synthesis work is only done using traditional DNA ,
peptide
synthesizers or PCRamplification or in vivo cloning. A large number of prepared
nucleic acid
probes, peptides, proteins and other biological macromolecules with special automated micro-dotting device to print at a higher density without interference to each other on specially treated glass, nylon film, nitrate cellulose membrane, and make it and support solidly combined. Supports need to be specially treated in advance, such as polylysine or aminosilane. These biomass molecules can also be attached to the support using other co-priced combination methods. More formed dot devices are now on sale, such as Biodot 's "print" meter and Cartesian Technologies 's Pix-Sys NQ/PAseries printer. These automated instruments suck biomass molecules out of the porous plate and "print" or "print" directly on the chip base based on the "print" or "print" needles they are equipped with. The "print" hour hand is in contact with the chip substitial and the "print" hour hand is kept at a distance from the chip base surface
. Therefore, the Print instrument is suitable for making higher density microarrays (e.g. 2500points /cm2 ), "printing" method Because of the large speckles of "printing", only lower density probe arrays can be formed, usually 400points /cm2
. The process of making chips by dot-like method is relatively simple and easy to master, the analytical equipment is easy to obtain, suitable for users to design micro-dot matrix flexibly according to their own needs, for scientific research and practical work.
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Today, most small and medium-sized companies use dot-like
technology to make biochips, in addition to individual companies such as Affymetrix , which has a reputation for
biochip development.
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