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    Home > Biochemistry News > Biotechnology News > E. coli DNA polymerase action and classification

    E. coli DNA polymerase action and classification

    • Last Update: 2020-11-03
    • Source: Internet
    • Author: User
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    related topicsRecombinant

    tool enzyme
    E. coli DNA polymerase (
    E.coli
    DNA polymerase) has three main effects: (1) 5' and 3' polymerization. But instead of copying chromosomes, it repairs DNA, filling gaps in DNA or leaving gaps left by the removal of RNA quotations. (2)3'-rarr;5' extase activity. Eliminates errors and nucleotides
    polymerization
    . (3) 5'-rarr;3' extase activity. Remove the damaged DNA. It is applied in nick translation.Klenow fragment of E. coli DNA polymerase I. is a fragment of the complete DNA polymerase I. only in 5'-rarr;3 polymerase activity and 3'-rarr;5' exocetic enzyme activity, lost 5'-rarr;3 exocetic enzyme activity. It can be used to fill the end of a single strand of DNA into a double strand. If a 32P-labeled triphosphate nucleotide is supplied, the DNA can be
    with a
    isotope. When a DNA fragment with a single-stranded sticky end is cut with a staggered cutting restriction enzyme, and the DNA fragments that are cut into flat-head ends are used, the single-stranded patch at the sticky end can be used first to make a flat head, and then the two DNA fragments can be connected under the action of
    DNA connection
    ase.is also E. coli
    DNA polymerase
    II. and E. coli DNA polymerase III. The former cannot use single-stranded DNA or poly (dA-dT) as a template. Magnesium ions and dNTP are required to exhibit enzyme activity and do not spontaneously synthesize DNA. The latter does not have the activity of 5'-rarr;3 exolytic enzymes, nor can single-stranded DNA be used as a template.
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